PCR Equipment and Supplies
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Thermo Scientific™ dITP Solution (100 mM)
Thermo Scientific dITP (2'-deoxyinosine 5'-triphosphate) is a highly stable nucleotide supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.
Thermo Scientific™ dUTP Solution (100 mM)
Thermo Scientific dUTP (2'-deoxyuridine 5'-triphosphate) is highly stable nucleotide, supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.
Thermo Scientific™ Aminoallyl-dUTP Solution (50 mM)
Thermo Scientific Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) is supplied as 10 mM and 50 mM aqueous solutions titrated to pH 7.0 with NaOH.
Applied Biosystems™ Eukaryotic 18S rRNA Endogenous Control (VIC™/MGB probe, primer limited)
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Eukaryotic 18S rRNA Endogenous Control (VIC™/MGB probe, primer limited)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ Anchored Oligo(dT)20 Primer
Anchored Oligo(dT)20 Primer is a primer mixture consisting of a string of 20 deoxythymidylic acid residues followed by dV (dG, dA, or dC) and then by dN (dA, dT, dG, or dC).
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ SuperScript™ IV One-Step RT-PCR System
The Invitrogen SuperScript One-Step RT-PCR System, available with or without ezDNase, combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Reverse Transcriptase | SuperScript IV |
| Final Product Type | PCR Amplified cDNA |
| Quantity | 1 Ea. |
| PCR Method | 1-step RT-PCR |
| Detection Method | Gel Electrophoresis |
| Polymerase | Platinum SuperFi |
| Format | Kit |
| Size (Final Product) | Up to 14 kb |
| Reaction Format | Master Mix |
| Reaction Speed | 10 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Hot Start | Built-In Hot Start |
| Optimal Reaction Temperature | 45°C to 60°C |
| Technique | 1-step RT-PCR |
| Starting Material | RNA |
Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq High Fidelity DNA Polymerase
The SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity is designed for convenient end-point detection and analysis of RNA molecules by one-step RT-PCR.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Reverse Transcriptase | SuperScript III |
| Final Product Type | PCR Amplified cDNA |
| PCR Method | 1-step RT-PCR |
| Detection Method | Gel Electrophoresis |
| Polymerase | Platinum Taq High Fidelity |
| Format | One-Step RT-PCR System |
| Size (Final Product) | Up to 10 kb |
| Reaction Format | Premixed Components |
| Reaction Speed | 30 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Hot Start | Built-In Hot Start |
| Optimal Reaction Temperature | 50°C |
| Technique | 1-Step RT-PCR |
| Starting Material | RNA |
Applied Biosystems™ Path-ID™ qPCR Master Mix
Ambion™ Path-ID™ qPCR Master Mix is a convenient enzyme, buffer, and dNTP mix for TaqMan™ probe-based, real-time PCR detection of DNA targets.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Purity or Quality Grade | UP (Ultra Pure) |
| Format | Tube |
| Passive Reference Dye | ROX (Pre-mixed) |
| Form | Liquid |
| Sample Type | DNA (Genomic), cDNA |
| Reaction Speed | Fast or Standard |
| Hot Start | Built-In Hot Start |
| For Use With (Application) | Animal Health,Pathogen Detection |
| PCR Method | qPCR |
| Product Line | Ambion, Path-ID |
| Detection Method | Primer-probe |
Invitrogen™ SuperScript™ III Platinum™ One-Step qRT-PCR Kit
Combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Taq DNA Polymerase |
| Product Type | One-Step qRT-PCR Kit |
| For Use With (Equipment) | 7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ |
| Sample Type | RNA |
| Reaction Speed | Standard |
| For Use With (Application) | Gene Expression,Pathogen Detection,Virus Detection |
| PCR Method | 1-step RT-qPCR |
| Product Line | Platinum, SuperScript |
| Detection Method | Primer-probe |
Applied Biosystems™ TaqPath™ 1-Step RT-qPCR Master Mix, CG
TaqPath 1-Step RT-qPCR Master Mix, CG, is designed for robust and reproducible one-step pathogen detection and gene expression workflows. The single-tube 4X formulation contains thermostable MMLV reverse transcriptase, dNTPs, UNG, ROX dye, and thermostable Fast DNA polymerase.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Reverse Transcriptase | M-MLV |
|---|---|
| Form | Liquid |
| InhibitorToleranceSimple | High |
| Concentration | 4X |
| For Use With (Application) | Microbial Detection,DNA Quantification,Presence/Absence |
| PCR Method | 1-step RT-qPCR |
| Multiplex Capability | Up to 3-plex |
| Detection Method | Primer-probe |
| Includes | dUTP, UNG/UDG |
| Thermostability | Not Applicable |
| Polymerase | Fast DNA Polymerase |
| Sensitivity | Down to 10 copies |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System, 7500 Fast Dx System, QuantStudio™ Dx |
| Sample Type | RNA, cDNA, DNA |
| Reaction Speed | Fast or Standard |
| Target Specificity | RNA only, Both RNA and DNA |
| Hot Start | Built-In Hot Start |
| Product Line | TaqPath |
Invitrogen™ Platinum™ PCR SuperMix High Fidelity
Platinum PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Wet or Dry Ice |
|---|---|
| Polymerase | Platinum Taq DNA Polymerase High Fidelity |
| GC-Rich PCR Performance | Low |
| Fidelity (vs. Taq) | 6X |
| Size (Final Product) | 15 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Reaction Speed | Standard |
| Hot Start | Built-In Hot Start |
| Overhang | Mixed |
| Concentration | 1.1X |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Applied Biosystems™ Human ACTB (Beta Actin) Endogenous Control (VIC™/MGB probe, primer limited)
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Human ACTB (Beta Actin) Endogenous Control (VIC™/MGB probe, primer limited)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ Mouse ACTB (Actin, Beta) Endogenous Control (FAM™ Dye/MGB probe, Non-Primer Limited)
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Mouse ACTB (Actin, Beta) Endogenous Control (FAM™ Dye/MGB probe, Non-Primer Limited)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ Taq DNA Polymerase, native
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Wet or Dry Ice |
|---|---|
| GC-Rich PCR Performance | Low |
| Fidelity (vs. Taq) | 1X |
| Overhang | 3'-A |
| Concentration | 5 U/μL |
| For Use With (Application) | Standard PCR |
| Exonuclease Activity | 5' - 3' |
| Polymerase | Taq DNA Polymerase |
| Format | Stand-alone enzyme |
| Size (Final Product) | 5 kb or less |
| Reaction Format | Separate Components |
| Product Type | DNA Polymerase |
| Reaction Speed | Standard |
| Hot Start | No |
| Starting Material | DNA |
Invitrogen™ Platinum™ SuperFi™ DNA Polymerase
Invitrogen Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Wet or Dry Ice |
|---|---|
| Polymerase | Platinum SuperFi DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 300X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Separate Components |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |